Abstract: [Objective] [Method] This study aims to explore the effects of artificial aging on seed vigor,starch granule,starch mass fraction and analyze the activity and endogenous hormones in the endosperm of barley seeds with high temperature and high humidity artificial aging treatment in the early stage of germination.[Result]The results showed that:The germination indexes and embryo vitality of 4 varieties decreased gradually with increasing aging time.Artificial aging increased the small starch granules and reduced large starch granules of endosperm in the germination 72 h.In addition to that,artificial aging increased starch mass fraction during the germination,reduced the activity of α-amylase and β-amylase of all barley and there were significant differences between the aging 15 d and the control.Content of GA3 was lower than that of CK in the germination 24 h but higher than that of the CK in the germination 48 to 72 h.However,contents of ABA were always higher than those of the CK in the all germination process.[Conclusion] The balance between GA3 and ABA was broken by aging,which resulted in the decrease of amylase activity and the slow-down of starch decomposition,so then barley seed vigor was decreased and the germination inhibited.