Abstract: [Objective] To study the cross pollination compatibility of different varieties in plum(Prunus domestica L.)and lay the foundation for the configuration and cross breeding of the European cross pollination varieties.[Method]Determination of Victoria of pollen number,pollen viability,fruit-setting rate and growth status of pollen tube of three Prunus domestica L.cv.Stanley,Prunus domestica L.cv.French and Prunus domestica L.cv.[Result]The pollen amount of single flower was Prunus domestica L.cv.Stanley 5.00 ten thousand grain/flower,Prunus domestica L.cv.French 3.12 ten thousand grain/flower and Prunus domestica L.cv.Victoria 1.45 ten thousand Grain/flower.Pollen viability was more than 20;,belonged to the medium-higher pollen viability;3 various of plum cultivars self-fertilization of fruit-setting rate,Prunus domestica L.cv.Victoria 0.47;,Prunus domestica L.cv.French 5.71;,Prunus domestica L.cv.Stanley 8.14;;3 plum cultivars pollination: the Prunus domestica L.cv.Victoria as the female parent,respectively in the Prunus domestica L.cv.French and Prunus domestica L.cv.Stanley as male parent,the fruit setting rate was respectively 5.88;,7.17;.Prunus domestica L.cv.Stanley,Prunus domestica L.cv.Victoria as female parent and male parent as the fruit setting rate was 12.15;,with Prunus domestica L.cv.French was a combination of male fruit rate was 6.34;.Prunus domestica L.cv.French as the female parent,Prunus domestica L.cv.Stanley and Prunus domestica L.cv.Victoria as male parent pollination,fruit setting rate were 11.49;,14.41;.When the plum and Prunus salicina L.cv.with Meilili as male parent after pollination,the fruit-setting rate was ≤ 0.05;,showing incompatibility of hybridization;the fluorescent display plum varieties mutual pollination showed affinity,self-fertilization was slow or abnormal pollen tube emerged,only a small amount of pollen tube went into the base of the style;the phenomenon of pollen tube bending or apex expansion after the hybridization of Prunus salicina L.cv.with Meilili hybrid appeared.[Conclusion]Prunus domestica L.cv.Stanley,Prunus domestica L.cv.French,Prunus domestica L.cv.Victoria and Prunus salicina L.cv.Meilili belonged to the middle-higher pollen vitality of varieties;plum cultivars self-fertilization of fruit-setting rate was relatively lower,so they need pollination tree;the pollen viability and stigma fluorescence microscopy,Prunus domestica L.cv.French and Prunus domestica L.cv.Stanley as pollination varieties to Prunus domestica L.cv Victoria,Prunus domestica L.cv Victoria as for Prunus domestica L.cv.French pollination varieties,Prunus domestica L.cv.Stanley appropriate pollination varieties Prunus domestica L.cv.Victoria and Prunus domestica L.cv.French;Prunus domestica L.cv.French×Prunus domestica L.cv.Victoria was better pollinators;Prunus salicina L.cv.Meilili was not fitted with the plum pollination.