Abstract: [Objective] Through conventional tissue culture propagation technology of the 4 new introduced varieties of German iris: Edith Wolford,Going My WaY,Red Zinger and October Sun,this project aims to explore the effective way of Iris germanica conventional and tissue culture in the hope of providing reliable technical support for the production of German iris seedlings propagation.[Method]In different seasons(spring and autumn),the survival rate,germination rate and effects on flowering with division propagation were studied about the four German irises,meanwhile the best hormone levels of explant induction,transgenerational and rooting medium were studied,and the rapid propagation system of each type was established.[Result]Using German irises of conventional breeding,the optimal points strains breeding time was after or before the start of root activity,after the fall dormancy or before the spring bud.2-3 shoots were transplanted in ramets.The effect of single plant transplanting on plant flowering was large,and flowering rate was low.The ramets with 5 buds were better than those with 3 buds,when German iris tissue culture propagation was carried out,its flower stems were used as explants and we could obtain directly adventitious bud on induction medium MS +BA 3.0-4.0 mg/L+NAA 1.0 mg/L,multiplication medium was MS + BA 1.0 mg/L + NAA 0.5 mg/L,with buds of 4-5,the propagation rate was more than 300;;rooting medium was 1/2 MS + NAA 0.5 mg/L with buds of 4-5,15 d vaccination,the rooting rate was 100;;The imported transplanting substrate was the best,and the transplanting survival rate can reach up to 100;.[Conclusion]Using German irises for conventional breeding,was after the fall dormancy or before the spring bud.2-3 shoots were transplanted in ramets.The effect of single plant transplanting on plant flowering was large,and flowering rate was low.When German iris tissue culture propagation was being carried on,we obtained directly adventitious bud on induction medium MS +BA 3.0-4.0 mg/L+NAA 1.0 mg/L,using stem section of flower organs as explant,multiplication medium was MS + BA 1.0 mg/L + NAA 0.5 mg/L,the propagation rate was more than 300;;rooting medium was 1/2 MS + NAA 0.5 mg/L;transplanting substrate to import crude plant was the best,and transplanting survival rate can reach up to 100;.