Abstract: [Objective] A high performance liquid chromatography (HPLC) method was developed to determine the concentration of four endogenous hormones [indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), zeatin (ZT)] in young fruit of Korla fragrant pear.[Method]After screening and optimization of conditions, 80; methanol was chosen as extraction solvent.Polyvinylpolypyrrolidone (PVPP) was used to absorb impurities.The samples were extracted with petroleum ether and ethyl acetate.The extracts were purified with a reverse pHase Sep-Pak C18 column (250 mm × 4.6 mm, 5 μm particle size).The column temperature was 40 ℃.The flow rate was 1 mL/min.The injection volume was 10 μL.The mobile phase was 45; methanol (A) and 55; acetic acid (B, pH=3.5).The detection wavelength was 254 nm.[Result]ZT, GA3, IAA, abscisic acid analysis method of high performance liquid chromatography was established.The HPLC recovery of known concentrations of IAA, ABA, GA3, and ZT ranged between 80.4; and 91.2;.The relative standard deviations (RSDs) ranged between 1.54; and 8.1;.The experimental results were stable and reliable.[Conclusion]The HPLC method developed in this study is simple, rapid, reproducible, and accurate.Therefore, it is suitable for rapid and accurate quantification of ZT, GA3, IAA and ABA in pear fruit.