Abstract: [Objective] To determine the optimum fertilization rate of microbial fertilizer of Fritillaria high yield and quality.[Method]Using the single factor experiment design, setting up 5 microbial fertilizer fertilization treatments respectively: 0, 2, 4, 6, 8 kg/ 667m2, during the development period of Fritillaria: germination stage, bud stage, flowering stage, the microbial fertilizer application rates were 10;, 20;, 30; and 40;, respectively.The plant height of Fritillaria, plant fresh weight and bulb fresh weight, yield and soil organic matter, total nitrogen and pH were measured and analyzed in this process.[Result]The results showed that with the increase of microbial fertilizer application rate, plant height, each period plant Fritillaria fresh weight and bulb fresh weight and yield of Fritillaria demonstrated first upward and then downward trend;With the increase of microbial fertilizer application rate, soil organic matter, soil total nitrogen content and soil pH did not change significantly during germination and early flowering stages.At the bud stage, except the soil organic matter and total nitrogen content, differences appeared in the rest elements.When the microbial fertilizer application amount was 4.29 kg/667 m2, the yield of Fritillaria reached the highest level, that was 538.55 kg/667 m2;during the germination stage, 0.29 kg/667 m2 caladium should be used, and bulb fresh weight was relatively heavy;at the beginning of flowering period, 1.02 kg/667 m2 Fritillaria should be used, and the bulb fresh weight was relatively heavy.[Conclusion]The microbial fertilizer as base fertilizer, ditching topdressing microbial fertilizer during the growth period, yield increase and efficiency of Fritillaria can be achieved.