]*>","")" /> Cloning of Phosphoserine Aminotransferase (SerB) Gene of Escherichia coli and Expression in Brevibacterium flavum
Xinjiang Agricultural Sciences

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Cloning of Phosphoserine Aminotransferase (SerB) Gene of Escherichia coli and Expression in Brevibacterium flavum

YIN Li-xia;CUI Chun-sheng;JIAN Zi-jian;TAN Hui-lin;BAO Hui-fang   

  • Received:2006-02-25 Revised:2006-02-25 Online:2006-02-25 Published:2006-02-25

大肠杆菌SerB基因的克隆及其在黄色短杆菌中的表达

殷丽霞;崔春生;简子健;谭慧林;包慧芳   

  1. 新疆农业大学动物医学学院,新疆乌鲁木齐,830052;新疆农科院微生物所,新疆乌鲁木齐,830000





摘要： 磷酸丝氨酸转氨酶(Phosphoserine aminotransferase,SerB)是L-丝氨酸生物合成中的关键酶,应用PCR技术从大肠杆菌JM109(E.Coli JM109)中扩增出磷酸丝氨酸转氨酶基因,将其与表达载体pEC 7连接.通过电转化方法,将重组质粒转入黄色短杆菌(Brevibacterium flavum C-11,BfC-11)中,经酶活检测和摇瓶发酵培养,含有重组表达质粒的BfC-11B的磷酸丝氨酸转氨酶的活力和L-丝氨酸产率均比原宿主菌BfC-11有所提高,为构建L-丝氨酸高产基因工程菌的研究奠定了基础.