巴尔喀什蘑菇子实体漆酶纯化与理化特性及对酚类化合物的降解能力

努尔孜亚·亚力买买提, 郝敬喆, 贾文捷, 陈浩宇, 罗影, 贾培松

Purification and Physicochemical Properties of Laccase from Fruiting Bodies of Agaricus balchaschensis

Nurziya Yarmamat, HAO Jingzhe, JIA Wenjie, CHEN Haoyu, LUO Ying, JIA Peisong

表1 野生巴尔喀什菇漆酶纯化变化

Table 1 Results of laccase purification from A. balchaschensis mushroom

分离方式 Separation mode 收集组分 Collect components 收集量 Collection volume(mg) 总活性 Total activity(U) 比活力 Specific activity (U/mg) 纯化倍数 Purification factor DEAE-cellulose Crude extract 4142.75 198.85 0.048 1 CM-cellulose cation exchange C1 19.89 1.01 0.051 1.06 C2 125.86 9.82 0.078 1.63 C3 363.31 68.30 0.188 3.92 Q-Sepharose anion exchange Q1 83.82 31.26 0.373 7.77 Superdex 75 SU1 6.4 108.93 17.02 354.58