Abstract: 【Objective】 To study the effects of nitrogen and water collaborative supply on accumulation and distribution of dry matter and nitrogen, nitrogen transport and use efficiency of maize hybrid Zhuyu 216. 【Methods】 Split plots experiments on two factors of nitrogen and irrigation were designed, and there were three irrigation levels: W₀ (0 m³/hm²), W₁ (750 m³/hm²) and W₂ (1,500 m³/hm²) and three nitrogen application levels: N₀ (0 kg/hm²), N₁(150 kg/hm²) and N₂(300 kg/hm²). 【Results】 The results showed that the accumulation of aboveground dry matter and nitrogen at maturity stage, and nitrogen absorption efficiency were the highest under nitrogen and water collaborative supply of W₂ water (1,500 m³/hm²) and N₁ nitrogen (150 kg/hm²), the collaborative supply of N₁ nitrogen (150 kg/hm²) and W₁ water (750 m³/hm²) was conducive to the accumulation of dry matter and nitrogen migration into grains, which increased the nitrogen transport amount in vegetative organs, enhanced the distribution proportion in grains, and raised nitrogen use efficiency, nitrogen transport efficiency, nitrogen transport contribution rate, nitrogen agronomic efficiency and nitrogen partial productivity. 【Conclusion】 Comprehensively considering the grain yield and fertilizer and water saving factors, the combination of irrigation amount 750 m³/hm² and nitrogen amount 150 kg/hm² was the optimal one for maize hybrid Zhuyu 216 based on the experiment results.