棉蚜取食被棉长管蚜危害棉花后其相关酶的活性

doi:10.6048/j.issn.1001-4330.2020.11.013

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (11): 2056-2064.DOI: 10.6048/j.issn.1001-4330.2020.11.013

棉蚜取食被棉长管蚜危害棉花后其相关酶的活性

吴娜, 张玉栋, 蔡晓虎, 史亚辉, 韩睿, 王俊刚

石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子 832003

收稿日期:2020-03-15 出版日期:2020-11-20 发布日期:2020-10-23
通信作者: 王俊刚(1970-),男,甘肃秦安人,教授,硕士生导师,研究方向为农业害虫与防治,(E-mail)wangjungang98@163.com
作者简介:吴娜(1990-),女,新疆石河子人,硕士研究生,研究方向为农业害虫与防治,(E-mail)275884611@qq.com
基金资助:
国家自然科学基金“棉长管蚜诱导棉花的防御反应对棉长管蚜和棉蚜种间竞争的影响”(31660519)

Studies on the Activities of Related Enzymes of Aphis gossypii after FeedingCotton Damaged by Acyrthosiphon gossypii

WU Na, ZHANG Yudong, CAI Xiaohu, SHI Yahui, HAN Rui, WANG Jungang

Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization/College of Agronomy, Shihezi University, Shihezi Xinjiang 832003, China

Received:2020-03-15 Online:2020-11-20 Published:2020-10-23
Correspondence author: WANG Jungang (1970-), male, native place: Taian Gansu, Professor and master tutor of Shihezi University, research field: plant protection, (E-mail) wangjungang98@ 163. com
Supported by:
Supported by Regional Science Foundation Project of the National Natural Science Foundation of China "Effects of Cotton Acyrthosiphon gossypii Inducing Cotton Defense Response on Interspecific Competition of Acyrthosiphon gossypii and Aphis gossypii" (31660519)

摘要/Abstract

摘要： 【目的】 研究棉蚜取食受蚜害胁迫棉花后,其体内酶活性的变化。【方法】 测定棉蚜取食受棉长管蚜危害24、48、72 h,及棉长管蚜、棉蚜交互危害的棉花后,其体内过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)、谷胱甘肽-S-转移酶(GST)活性。【结果】 棉蚜取食经棉长管蚜危害的棉花后,体内过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)、谷胱甘肽-S-转移酶(GST)活性均呈上升趋势。除SOD在棉长管蚜危害48 h后达到最大值86.03 U/mg,CAT、POD、AChE、GST均在棉长管蚜危害72 h后达到最大值,分别为452.82、8.12、0.080 9和52.24 U/mg。棉长管蚜和棉蚜交互危害中,2种蚜虫取食次序和取食时间对后取食棉蚜体内酶活性变化影响较大。第1次棉长管蚜危害对后取食棉蚜CAT、SOD、AChE、GST活性变化产生显著影响,第2次棉蚜危害对后取食棉蚜CAT、POD活性产生显著影响,第3次棉长管蚜危害对后取食棉蚜POD、AChE活性产生显著影响。对CAT活性影响最大的处理为:棉长管蚜-棉蚜-棉长管蚜依次危害72 h-72 h-24 h;对POD活性影响最大的处理为:棉长管蚜-棉蚜-棉长管蚜依次危害72 h-72 h-48 h;对SOD活性影响最大的处理为:棉长管蚜-棉蚜-棉长管蚜依次危害48 h-24 h-72 h;对AChE活性影响最大的处理为:棉长管蚜-棉蚜-棉长管蚜依次危害24 h-72 h-48 h;对GST活性影响最大的处理为:棉长管蚜-棉蚜-棉长管蚜依次危害72 h-48 h-48 h。【结论】 棉长管蚜危害棉花 72 h,对其后取食棉蚜体内酶活性影响最大;棉长管蚜和棉蚜交互危害中,第1次棉长管蚜危害对后取食棉蚜酶活性影响最大,第2次棉蚜危害和第3次棉长管蚜危害对后取食棉蚜酶活性影响均较小。

关键词: 棉长管蚜; 棉蚜; 保护酶; 解毒酶

Abstract: 【Objective】 To clarify the changes in enzyme activity of A. gossypii after eating cotton harmed by aphids .【Methods】 Catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), acetylcholinesterase (AChE) and glutathione-S-transferase (GST) activities in A. gossypii were determined after it was fed on cotton induced by Ac. gossypii for 24 h, 48 h and 72 h.【Results】 A. gossypii after eating cotton induced by Ac. gossypii, Catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), acetylcholinesterase (AChE), and glutathione-S-transferase (GST) activities showed upward trend. Except that SOD reached a maximum of 86.03 U/mg after 48h of Ac. gossypii induction,CAT, POD, AChE, and GST all reached their maximum values after 72 hours of induction of Ac. gossypii, which were 452.82 U/mg, 8.12 U/mg, 0.080,9 U/mg, and 52.24 U/mg, respectively. In the mutual induction of Ac. gossypii and A. gossypii, the feeding order and time of the two aphids had a great influence on the changes of enzyme activity in the later feeding of A. gossypii. The specific performance was: Cotton for the first time induced by Ac. gossypii had significant effects on the changes of CAT, SOD, AChE, and GST activities of A. gossypii that were fed later. The second A. gossypii induced cotton significantly affected the CAT and POD activities of the A. gossypii; The third Ac. gossypii induced cotton had a significant effect on the POD and AChE activities of A. gossypii in late feeding. The treatments of Ac. gossypii and A. gossypii that interacted to induce cotton to have the greatest effect on the five enzymes activities of A. gossypii that was fed later were as follows: treatment that had the greatest effect on CAT activity of A. gossypii was: Ac. gossypii-A. gossypii -Ac. gossypii were induced in sequence for 72 h-72 h-24 h; The treatment that had the greatest effect on POD activity of A. gossypii was: Ac. gossypii -A. gossypii -Ac. gossypii were induced in sequence for 72 h-72 h-48 h; The treatment that had the greatest effect on the SOD activity of A. gossypii was: Ac. gossypii -A. gossypii-Ac. gossypii were induced in sequence for 48 h-24 h-72 h; The treatment that had the greatest effect on the AChE activity of A. gossypii was: Ac. gossypii -A. gossypii -Ac. gossypii were induced in sequence for 24 h-72 h-48 h; The treatment that had the greatest effect on the GST activity of A. gossypii was: Ac. gossypii -A. gossypii -Ac. gossypii were induced in sequence for 72 h-48 h-48 h.【Conclusion】 Acyrthosiphon gossypii has the greatest effect on the enzyme activity of Aphis gossypii after feeding Ac. gossypii induced cotton for 72 hours; In the interactive induction of Ac. gossypii and A. gossypii,Ac. gossypii induced cotton for the first time, it has the greatest effect on the enzyme activity of A. gossypii in late feeding. The effects of the second A. gossypii induced cotton and the third Ac. gossypii induced cotton on the enzyme activity of A. gossypii were relatively small.

Key words: Acyrthosiphon gossypii; Aphis gossypii; protective enzymes; detoxifying enzymes

中图分类号:

S435.622

吴娜, 张玉栋, 蔡晓虎, 史亚辉, 韩睿, 王俊刚. 棉蚜取食被棉长管蚜危害棉花后其相关酶的活性[J]. 新疆农业科学, 2020, 57(11): 2056-2064.

WU Na, ZHANG Yudong, CAI Xiaohu, SHI Yahui, HAN Rui, WANG Jungang. Studies on the Activities of Related Enzymes of Aphis gossypii after FeedingCotton Damaged by Acyrthosiphon gossypii[J]. Xinjiang Agricultural Sciences, 2020, 57(11): 2056-2064.

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棉蚜取食被棉长管蚜危害棉花后其相关酶的活性

Studies on the Activities of Related Enzymes of Aphis gossypii after FeedingCotton Damaged by Acyrthosiphon gossypii

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