新疆农业科学 ›› 2020, Vol. 57 ›› Issue (7): 1349-1360.DOI: 10.6048/j.issn.1001-4330.2020.07.020

• • 上一篇    下一篇

亮斑扁角水虻形态观察及诱产卵菌的筛选

荆海全1.2, 钟问1, 詹发强2, 杨蓉2, 包慧芳2, 侯敏2, 龙宣杞2   

  1. 1.新疆大学生命科学与技术学院,乌鲁木齐 830052;
    2.新疆农业科学院微生物应用研究所,乌鲁木齐 830091
  • 收稿日期:2019-08-16 出版日期:2020-07-20 发布日期:2020-06-22
  • 通信作者: 龙宣杞(1969-),女,四川人,研究员,硕士生导师,研究方向为农业微生物资源及利用,(E-mail)609160628@qq.com
  • 作者简介:荆海全(1993-),男,甘肃人,硕士研究生,研究方向为农业废弃物资源化利用,(E-mail)1352927576@qq.com
  • 基金资助:
    自治区科技支疆项目(2017E0250);自治区引进高层次柔性人才引进项目(唐雪明,2017年)

Morphological Observation of Hermetia illucens and Preliminary Screening of Oviposition-inducing Bacteria

JING Haiquan1,2, ZHONG Wen1, ZHAN Faqiang2, YANG Rong2, BAO Huifang2, HOU Min2, LONG Xuanqi2   

  1. 1. College of Life Science and Technology, Xinjiang University, Urumqi 830052, China;
    2. Research Institute of Applied Microbiology, Xinjiang Academy of Agriculture Sciences, Urumqi 830091,China.
  • Received:2019-08-16 Online:2020-07-20 Published:2020-06-22
  • Correspondence author: Long Xuanqi(1969-),woman,Sichuan province,researcher, research direction: Agricultural microbial resources and utilization,(E-mail)609160628@qq.com

摘要: 【目的】 研究引进亮斑扁角水虻在室内饲喂条件下的生活史、成虫种群雌雄比例变化规律,对其卵表及幼虫肠道中诱成虫产卵菌进行筛选和鉴定,为亮斑扁角水虻在本地区的规模养殖和综合利用提供理论依据。【方法】 引进亮斑扁角水虻,在室内光照条件下饲喂4代,用直接观察法记录亮斑扁角水虻在不同饲喂条件下的生活史和生活习性,显微拍照记录水虻各龄期的形态特征;用稀释涂布平板及平板划线的方法,对亮斑扁角水虻卵携带菌及其幼虫肠道菌进行分离纯化,筛选可诱导产卵的菌株,并鉴定。【结果】 与内地相比,亮斑扁角水虻在新疆饲养周期稍有延长,但其发育完好,能够在本地区进行世代饲养;观察并记录了其卵、幼虫、蛹、成虫及其其生殖器形态;筛选到对亮斑扁角水虻雌性成虫有诱导产卵效果的两株菌e1Y3,其发酵液处理组平均产卵量分别为对照组的6.26和7.40倍,两株菌皆为革兰氏阳性菌,16S RNA测序初步鉴定e1为暹罗芽孢秆菌,Y3为节杆菌属,且Y3为1种潜在的新菌。【结论】 引进亮斑扁角水虻在新疆能够周年循环养殖,具有规模生产及综合开发利用潜力。

关键词: 亮斑扁角水虻; 形态特征; 诱产卵菌

Abstract: 【Objective】 To ascertain the life history and the change rule of male-female ratio of adult Hermetia illucens population under indoor feeding conditions, screen and preliminary identify oviposition-inducing bacteria from the egg surface and larval intestinal tract in the hope providing theoretical basis for the large-scale breeding and comprehensive utilization of Hermertia illucens in local area.【Method】 Hermetia illucen introduced has been reared 4 generations indoors, its life span and life habits in different diets were recorded by direct observation methods, morphological character was observed with microscope, oviposition-inducing bacteria from the egg surface and larval intestinal tract were separated and purified by dilute coating flat plate and flat plate scribing method, and were screened and preliminary identified as the bacteria afterwards.【Result】Compared with interior area, the period from eggs to adults was slightly prolonged slightly in Xinjiang, but it was developed well and could be reared for generations in this region. Eggs, larvae, pupae, adults and their genital morphology were observed and recorded. Two strains of bacteria named e1 and Y3 with oviposition-inducin effect were screened. The fermentation broth treatment could increase oviposition rate by 6.26 times and 7.40 times respectively compared with the control. The two strains have been tested as Gram-positive bacteria by Gram staining, and preliminary identified as Bacillus siamensis and Athrobacter respectively with methods of 16S RNA sequencing. Y3 was regarded as a potential new bacteria.【Conclusion】 Annual circular aquaculture of Hermetia illucens in Xinjiang is feasible. Hermetia illucens also has the potential of large-scale production and comprehensive development and utilization.

Key words: Hermetia illucens; morphological character; oviposition-introducing bacteria

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
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