预处理及培养方法协同作用对新铁炮百合种子萌发的影响

doi:10.6048/j.issn.1001-4330.2020.07.012

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (7): 1276-1286.DOI: 10.6048/j.issn.1001-4330.2020.07.012

预处理及培养方法协同作用对新铁炮百合种子萌发的影响

吴小媚, 袁延平, 魏肖如, 曹福祥, 陈己任, 吕长平, 李玉帆

湖南农业大学园艺园林学院/湖南省中亚热带优质花木繁育与利用工程技术研究中心,长沙 410128

收稿日期:2019-11-19 出版日期:2020-07-20 发布日期:2020-06-22
通信作者: 李玉帆(1989-),女,讲师,博士,研究方向为园林植物遗传育种,(E-mail)shangnv0797@163.com
作者简介:吴小媚(1996-),女,硕士研究生,研究方向为园林植物遗传育种,(E-mail)812004699@qq.com
基金资助:
湖南省自然科学基金(2019JJ50218);湖南农业大学青年科学基金(17QN30);湖南省研究生科研创新项目(CX20190517);湖南省科技计划项目经费资助(2018TP2007)

Synergistic Effect of Pretreatment and Cultural Method on Lilium ×fomolongi Germination

WU Xiaomei, YUAN Yanping, WEI Xiaoru, CAO Fuxiang, CHEN Jiren, LÜ Changping, LI Yufan

Hunan Mid-Subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center, College of Horticulture, Hunan Agricultural University, Changsha 410128, China

Received:2019-11-19 Online:2020-07-20 Published:2020-06-22
Correspondence author: LI Yufan(1989-),women,doctor,lecturer.Main research:Garden plant genetics and breeding,(E-mail)shangnv0797@163.com.

摘要/Abstract

摘要： 【目的】 研究不同预处理方式下培养方法协同作用对新铁炮百合种子萌发的影响。【方法】 以新铁炮百合雷山1号种子为材料,采用三因素四水平正交试验设计,研究不同水平的预处理方式、培养温度、培养方式对新铁炮百合雷山1号种子萌发的影响,以及不同温度下种子萌发过程中可溶性糖和过氧化物歧化酶(SOD)的动态变化规律。【结果】 培养温度是影响新铁炮百合雷山1号种子萌发的主要因素,其次是预处理方式和培养方式。雷山1号种子的最佳预处理方式是4℃浸种7 d,最佳培养温度是21℃,最佳培养方式是土壤播种,发芽率为99.18%,播种后第9 d发芽指数为4.65。不同温度处理下,种子萌发过程中超氧化物歧化酶(SOD)活性表现出先增加后下降的趋势,可溶性糖含量则呈现出先下降再上升的趋势,其中21℃处理下种子的抗逆性和贮藏物质的转化利用要高于15、18和25℃处理下的种子,21℃为适宜温度。【结论】 雷山1号种子的最佳处理方式为4℃浸种7 d于21℃下土壤播种。

关键词: 新铁炮百合; 种子萌发; 预处理方式; 培养方法; 正交试验

Abstract: 【Objective】 Lilium formolongi Raizan No.1 was used as experimental material, the orthogonal experiment design of three factors and four levels was used to research the effects of different seed pretreatments and cultural temperatures, and cultural method on Lilium formolongi Raizan No.1 seed germination. This experiment also researched the dynamic change of soluble sugars and superoxide dismutase (SOD) in different temperatures. 【Results】 The results showed that the key factor was cultural temperature, followed by pretreatment and cultural method. And as for Raizan No.1, the best way of pretreatment was soaking seed in 4℃ for 7 days, the optimum temperature was 21℃,the optimum culture mode was sowing in soil, its germination was 99.18% and the germination index was 4.65. At different temperatures, the activity of superoxide dismutase (sod) increased first and then decreased at germination stage, but the soluble sugar content showed a tendency of going down first and then up. Among them, the resistance of the seeds under 21℃ treatment and the conversion utilization of the stored substances were higher than those under 15℃,18℃ and 25℃ treatment, which confirmed that 21℃ was the appropriate temperature. 【Conclusion】 The optimum treatment of the Rayshan 1seeds was to sow at 21℃ for 4℃ for 7 days .

Key words: Lilium formolongi ; seed germination ; pretreatment ; cultural method; orthogonal experiment

中图分类号:

S681.9

吴小媚, 袁延平, 魏肖如, 曹福祥, 陈己任, 吕长平, 李玉帆. 预处理及培养方法协同作用对新铁炮百合种子萌发的影响[J]. 新疆农业科学, 2020, 57(7): 1276-1286.

WU Xiaomei, YUAN Yanping, WEI Xiaoru, CAO Fuxiang, CHEN Jiren, LÜ Changping, LI Yufan. Synergistic Effect of Pretreatment and Cultural Method on Lilium ×fomolongi Germination[J]. Xinjiang Agricultural Sciences, 2020, 57(7): 1276-1286.

参考文献

[1]孙红梅,贾子坤,陆阳,等.百合鳞片扦插繁殖的研究进展[J].北方园艺,2009,(2):141-146.
SUN Hongmei, JIA Zikun, LU Yang, et al. Advances on the Cutting Propagation of Scale in Lilium [J]. Northern Horticulture, 2009,(2):141-146.
[2] 李金鹏,郑春雨,刘井莉.百合属植物的研究进展[J].北方园艺,2013,(7):197-200.
LI Jinpeng, ZHENG Chunyu, LIU Jingli, et al. Advances in studies on lilium [J]. Northern Horticulture, 2009,(2):141-146.
[3] Okazaki, K. Lilium Species Native to Japan, and Breeding and Production Of Lilium In Japan[J]. Acta Horticulturae, 1996(414):81-92.
[4]韩秀丽,田晓明,贾桂霞.新铁炮百合单倍体植株的诱导[J].园艺学报,2010,37(2):263-268.
HAN Xiuli, TIAN Xiaoming, JIA Guixia, et al. Induction of Haploid Plantlets for Lilium × formolongi [J]. Acta Horticulture Sinica, 2010, 37(2):263-268.
[5]王新颖,李智辉,邢致远,等.新铁炮百合种子萌发的研究[J].种子,2006,(9):14-16.
WANG Xinying, LI Zhihui, XING Zhiyuan, et al. Study on Seed Germination of L.×L. formolongi [J].Seed, 2006,(9):14-16.
[6] 郝渊鹏,李浩铮,刘笑笑,等.预处理和播种方式对朱丽叶种子萌发的影响[J].山西农业科学,2016,44(12):1785-1788,1792.
HAO Yuanpeng, LI Zhenghao, LIU Xioaxiao, et al. Effects of Preliminary Treatment Methods and Sowing Way on Germination of Lilium formolongi Julius Seeds [J]. Shangxi Agricultural Sciences, 2016,44(12):1785-1788,1792.
[7] 韩秀丽,贾桂霞.新铁炮百合不同品种种子适宜萌发条件的研究[J].林业科学研究,2008,(5):729-733.
HAN Xiuli, JIA Guixia. Studies on Optimal Germination Conditions of Different Cultivars and Heat-resistance Techniques for Lilium × formolongi [J].Forest Research, 2008,(5):729-733.
[8] 孔祥生. 植物生理学实验技术[M]. 北京:中国农业出版社2008.
KONG Xiangsheng. Experiment techniques for plant physiology [M]. Beijing: China Agriculure Press. 2008.
[9] 王友凤. 福建青冈种子萌发的生理生态学机制研究[D].福州:福建农林大学,2007.
WANG Youfeng. The study on Ecophysiology Mechanism of seed of Cyclobalanopsis chungii [D].Fuzhou: Fujian Agriculture and Forestry University, 2007.
[10] 徐亮,包维楷,何永华.种子贮藏物质变化及其贮藏生理[J].种子,2003,(5):61-64.
XU Liang, BAO Weikai, HE Yonghua, et al. Changes of seed storage substance and plant physiology [J].Seed, 2003,(5):61-64.
[11] 宋倩,李志辉,童方平,等.黧蒴栲种源种子发芽的生理生化特性研究[J].中国农学通报,2013,29(7):6-11.
SONG Qian, LI Zhihui, TONG Fangping, et al. Physiological and Biochemical Characteristics in Seed Germination of Castanopsis fissa Rehd.et Wils [J].Chinese Agricultural Science Bulletin, 2013,29(7):6-11.
[12]Johnson T R , Kane M E , Hector E. Pérez. Examining the interaction of light, nutrients and carbohydrates on seed germination and early seedling development of Bletia purpurea (Orchidaceae)[J]. Plant Growth Regulation, 2011, 63(1):89-99.
[13]洪波.百合花卉的研究综述[J].东北林业大学学报,2000,(2):68-70.
HONG Bo. A review of studies on lily flower [J].Journal of Northeast Forestry University, 2000,(2):68-70.
[14]何泽明,温文兴,王凤兰,等.新铁炮百合种子发芽技术研究[J].广东农业科学,2011,38(16):33-35.
HE Zeming, WEN Wenxing, WANG Fenglan, et al. Study on seed germination of Lilium formolongi [J].Guangdong Agricultural Sciences, 2011,38(16):33-35.
[15]何丽萍,李龙根,庞祖云.野生泸定百合种子休眠和萌发习性的研究[J].种子,2010,29(4):46-49.
HE Liping, LI Longgen, PANG Zuyun, et al. Study on Dormancy and Germination Characters of Wild Lily (Lilium sargentiae Wilson) Seed [J]. Seed,2010,29(4):46-49.
[16]解雪华. 不同处理方式对百合种子萌发特性的影响[A]. 中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心.中国观赏园艺研究进展2015[C].中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心:中国园艺学会,2015:5.
XIE Xuehua. Effects of different treatment on germination characteristics of lily seeds [A].Ornamental horticulture committee of Chines horticultural society,National flower engineering technology research center. Advances ornamental horticulture in China 2015 [C].Ornamental horticulture committee of Chines horticultural society, National flower engineering technology research center: Chinese horticultural society, 2015:5.
[17]杨炜茹,张启翔.岷江百合种子萌发的研究[J].种子,2008,(11):5-7.
YANG Weiru, ZHANG Qixiang. Study on the seed Germination of Lilium regala [J].Seed, 2008,(11):5-7.
[18]崔祺,贾桂霞.3种百合组培快繁体系的优化[J].湖南农业大学学报(自然科学版),2014,40(6):621-626.
CUI Qi, JiA Guixia. Optimization of tissue culture and rapid propagation system for Lilium [J].Journal of Hunan Agricultural University (Natural Science Ed.), 2014, 40(6):621-626.
[19]辛霞,景新明,孙红梅,等.孑遗植物水杉种子萌发的生理生态特性研究[J].生物多样性,2004,(6):572-577.
XIN Xia, JING Xinming, SUN Hongmei, et al. Ecophysiological characteristics of seed germination of the relict plant Metasequoia glyptostroboide [J].Biodiversity, 2004,(6):572-577.
[20]胡晋. 种子生物学[M].北京:高等教育出版社, 2006.
HU Jing. Seed Biology [M]. Beijing: Higher Education Press. 2006.
[21]Koyro H W, Eisa S S. Effect of salinity on composition, viability and germination of seeds of Chenopodium quinoa Wild [J]. Plant & Soil, 2008, 302(1/2):79-90.

预处理及培养方法协同作用对新铁炮百合种子萌发的影响

Synergistic Effect of Pretreatment and Cultural Method on Lilium ×fomolongi Germination

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