117份小麦种质中5个矮秆基因的分子检测

doi:10.6048/j.issn.1001-4330.2019.08.001

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (8): 1373-1381.DOI: 10.6048/j.issn.1001-4330.2019.08.001

• 作物遗传育种·物质资源·分子生物学 • 下一篇

117份小麦种质中5个矮秆基因的分子检测

陈向东, 吴晓军, 胡喜贵, 李淦, 姜小苓, 李笑慧, 李小利, 胡铁柱, 茹振钢

河南科技学院小麦中心/河南省杂交小麦重点实验室/现代生物育种河南省协同创新中心,河南新乡 453003

收稿日期:2019-04-22 出版日期:2019-08-20 发布日期:2019-10-10
通信作者: 茹振钢(1958-),男,河南沁阳人,教授,研究方向为小麦遗传育种,(E-mail)rzgh58@163.com
作者简介:陈向东(1982-),男,河南偃师人,讲师,博士,研究方向为小麦遗传育种,(E-mail)xdchen716@126.com
基金资助:
国家自然科学基金(31701502);河南省高等学校重点科研项目(16A210047);新乡市科技重大专项(ZD17005)

Molecular Detection of Five Dwarf Genes in 117 Wheat Germplasm Resources

CHEN Xiang-dong, WU Xiao-jun, HU Xi-gui, LI Gan, JIANG Xiao-ling, LI Xiao-hui, LI Xiao-li, HU Tie-zhu, RU Zhen-gang

Wheat Center, Henan Institute of Science and Technology, Henan Key Laboratory of Hybrid Wheat, Henan Modern Biological Breeding Collaborative Innovation Center, Xinxiang Henan 453003,China

Received:2019-04-22 Online:2019-08-20 Published:2019-10-10
Correspondence author: RU Zhen-gang (1958-), male, Qinyang, Henan Province, professor, research direction is wheat genetic breeding research, (E-mail) rzgh58@163.com
Supported by:
Supported by the National Natural Science Foundation of China (31701502); Key Scientific Research Projects of Henan University (16A210047); Major Scientific and Technological Project of Xinxiang City (ZD17005)

摘要/Abstract

摘要： 【目的】研究我国小麦主产区部分小麦品种中矮秆基因的分布,为发掘具产量潜力且利用较少的矮秆基因提供种子依据。【方法】利用5个矮秆基因分子标记,对我国小麦主产区117份小麦种质进行检测。【结果】117份种质材料中,矮秆基因分布频率依次为Rht8(68份,58.1%)>Rht-B1b(56份,47.9%)>Rht-D1b(46份,39.3%)> Rht9(18份,15.4%)> Rht13(14份,12.0%)。部分种质中同时携带2个矮秆基因的组合占到37.8%;同时携带3个及以上基因的组合占到21.4%,其中以同时携带Rht-B1b、Rht-D1b和Rht8基因种质最多,占到12.8%。5种矮秆基因均不携带的种质有15份,占到12.8%。【结论】鉴定出14份携带Rht13矮秆基因的新种质。

关键词: 小麦; 矮秆基因; 分子标记; 株高

Abstract: 【Objective】 To analyze the distribution of dwarf genes in wheat cultivars from main wheat producing areas of China and to screen dwarf genes with yield potential.【Method】117 wheat germplasm Resources were detected by five molecular markers of dwarf genes.【Result】The results showed that among 117 germplasm materials, the distribution frequency of dwarf gene was Rht 8 (68, 58.1%) > Rht-B1b (56, 47.9%) > Rht-D1b (46, 39.3%) > Rht 9 (18, 15.4%) > Rht 13 (14, 12.0%). 37.8% of germplasms contained two dwarf genes. 21.4% of the germplasms carried three or more genes at the same time, of which, 12.8% carried Rht-B1b, Rht-D1b and Rht-8 genes. In addition, the five dwarf genes were not detected in 15 germplasms (12.8%).【Conclusion】These results provide theoretical basis and material support for wheat breeding in China.

Key words: wheat; dwarf genes; molecular markers; plant height

中图分类号:

S512

陈向东, 吴晓军, 胡喜贵, 李淦, 姜小苓, 李笑慧, 李小利, 胡铁柱, 茹振钢. 117份小麦种质中5个矮秆基因的分子检测[J]. 新疆农业科学, 2019, 56(8): 1373-1381.

CHEN Xiang-dong, WU Xiao-jun, HU Xi-gui, LI Gan, JIANG Xiao-ling, LI Xiao-hui, LI Xiao-li, HU Tie-zhu, RU Zhen-gang. Molecular Detection of Five Dwarf Genes in 117 Wheat Germplasm Resources[J]. Xinjiang Agricultural Sciences, 2019, 56(8): 1373-1381.

参考文献

[1] Hedden, P. (2003). The genes of the green revolution. Trends in Genetics, 19(1):5-9.
[2] Dzhelepov, K. (1970). Short-strawed mutants of winter wheat induced by means of x irradiation and ethyl methanesulphonate. Comptes Rendus De Lacademie Des Sciences Agricoles En Bulgarie, 361-368.Viglasi P. Short-strawed mutants of Karcag 522 winter wheat induced by gamma rays [J]. Acta Agronomica Academiae Scientiarum Hungaricae, 1968, 17(1-2): 205-&.
[3] Morris, R., Schmidt, J. W., & Johnson, V. A. (1972). Chromosomal location of a dwarfing gene in 'tom thumb' wheat derivative by monosomic analysis1. Crop Science, 12(2):247-249.
[4] Peng, Z. S., Li, X., Yang, Z. J., & Liao, M. L. (2011). A new reduced height gene found in the tetraploid semi-dwarf wheat landrace aiganfanmai. Genetics and molecular research: GMR,10(4): 2,349-2,357.
[5] Chen, G., Zheng, Q., Bao, Y., Liu, S., Wang, H., & Li, X. (2012). Molecular cytogenetic identification of a novel dwarf wheat line with introgressedthinopyrum ponticumchromatin. Journal of Biosciences, 37(1):149-155.
[6] Miralles, D. J. , & Slafer, G. A. (2010). Yield, biomass and yield components in dwarf, semi‐dwarf and tall isogenic lines of spring wheat under recommended and late sowing dates. Plant Breeding, 114(5):392-396.
[7] Tobias Würschum, Langer, S. M. , & Longin, C. F. H. (2015). Genetic control of plant height in european winter wheat cultivars. Theoretical and Applied Genetics., 128(5):865-874.
[8] Sial, M. A. , Arain, M. A. , Javed, M. A. , & Jamali, K. D. (2002). Genetic impact of dwarfing genes (rht1 and rht2) for improving grain yield in wheat. Asian Journal of Plant Sciences, 1(3):254-256.
[9] Flintham, J., E., Angus, W., J., Gale, & M., D. (2000). Heterosis, overdominance for grain yield, and alpha-amylase activity in F1 hybrids between near-isogenic Rht dwarf and tall wheats. Journal of Agricultural Science, 129(4):371-378.
[10] Richards, R. A. (1992). The effect of dwarfing genes in spring wheat in dry environments. i. agronomic characteristics. Australian Journal of Agricultural Research, 43(3): 517-527.
[11] Richards, & R. A. (1992). The effect of dwarfing genes in spring wheat in dry environments. II. growth, water use and water-use efficiency. Australian Journal of Agricultural Research, 43(3): 529-539.
[12]李杏普, 刘玉平, 郭文奇, 等. 不同Rht基因对小麦子粒品质的影响[J]. 华北农学报, 2001, 16(1):18-22.
LI Xing-pu,LIU Yu-ping,GUO Wen-qi,et al. (2001). Effects of Different Rht Genes on Wheat Grain Quality [J]. Acta Agriculturae Boreali-Sinica, 16(1):18-22. (in Chinese)
[13] Linn, T. Z. , Bachir, D. G. , Chen, L. , & Hu, Y. G. (2017). Effects of gibberellic acid responsive dwarfing gene rht9 on plant height and agronomic traits in common wheat, 5(4):102.
[14] Rebetzke, G. J., Ellis, M. H., Bonnett, D. G., Condon, A. G., Falk, D., & Richards, R. A. (2011). The rht13 dwarfing gene reduces peduncle length and plant height to increase grain number and yield of wheat. Field Crops Research, 124(3):323-331.
[15]慕美财, 刘勇, 郭小丽, 等. 山东小麦品种中矮秆基因Rht-B1b、Rht-D1b分布的分子鉴定[J]. 分子植物育种, 2005,(4):473-478.
MU Mei-cai, LIU Yong, GUO Xiao-li, et al. (2005) Distribution of Rht-Blb and Rht-Dlb in Wheat Cultivars in Shandong Detected by Molecular Markers [J]. Molecular Plant Breeding, (4):473-478. (in Chinese)
[16]杨芳萍, 夏先春, 张勇, 等. 春化、光周期和矮秆基因在不同国家小麦品种中的分布及其效应[J]. 作物学报, 2012, 38(7):1 155-1 166.
YANG Fang-ping, XIA Xian-chun, ZHANG Yong, et al. (2012). Distribution of Allelic Variation for Vernalization, Photoperiod, and Dwarfing Genes and Their Effects on Growth Period and Plant Height among Cultivars from Major Wheat Producing Countries [J]. Acta Agronomica Sinica, 38(7):1,155-1,166. (in Chinese)
[17]唐娜,李博,闵红,等.分子标记检测矮秆基因Rht-B1b、Rht-D1b和Rht8在我国小麦中的分布[J].中国农业大学学报,2012,17(4):21-26.
TANG Na, LI Bo, MIN Hong, et al. (2012). Distribution of dwarfing genes Rht-B 1b, Rht-D 1b and Rht8 in Chinese bread wheat cultivars detected by molecular markers [J].Journal of China Agricultural University, 17(4):21-26. (in Chinese)
[18]孙树贵, 李艳丽, 鲁敏, 等. 67份美国小麦品种矮秆基因的分子标记检测[J]. 麦类作物学报, 2013,33(6):1 087-1 092.
SUN Shu-gui, LI Yan-li, LU Min, et al. (2013)Distribution of Dwarfing Genes in 67 American Wheat Cultivars Detected by Molecular Markers [J]. Journal of Triticeae Crops, 33(6):1,087-1,092. (in Chinese)
[19]昝凯, 李春游, 敬樊, 等. 部分印度小麦品种矮秆基因的检测及其对部分性状的影响[J]. 麦类作物学报, 2015,35(7):910-917.
ZAN Kai, LI Chun-you, JING Fan, et al.( 2015) Detection of Dwarfing Genes in Some India Wheat Cultivars and Their Influences on Partial Agronomic Characteristics [J].Journal of Triticeae Crops,35(7):910-917. (in Chinese)
[20]王玉叶, 张海萍, 来得娥, 等. 257份小麦品种资源中矮秆基因的分子检测[J]. 安徽农业大学学报, 2013,40(5):860-866.
WANG Yu-ye, ZHANG Hai-ping, LAI De-e, et al. (2013)Detection of dwarf genes in 257 wheat variety resources using molecular markers [J]. Journal of Anhui Agricultural University, 40(5):860-866. (in Chinese)
[21]钟明志, 魏淑红, 彭正松, 等. 小麦Rht矮秆基因研究和应用综述[J]. 分子植物育种, 2018, 16(20):144-151.ZHONG Ming-zhi, WEI Shu-hong, PENG Zheng-song, et al.( 2018)A Review of the Research and Application of Rht Dwarf Genes in Wheat [J]. Molecular Plant Breeding, 16(20):144-151. (in Chinese)
[22]张德强, 宋晓朋, 冯洁, 等. 黄淮麦区小麦品种矮秆基因Rht-B1b、Rht-D1b和Rht8的检测及其对农艺性状的影响[J]. 麦类作物学报, 2016, 36(8):975-981.
ZHANG De-qiang, SONG Xiao-ming, FENG Jie, et al.( 2016) Detection of Dwarf Genes Rht-B1b, Rht-D1b and Rht8 in Huanghuai Valley Winter Wheat Areas and Their Influences on Agronomic Characteristics[J].Journal of Triticeae Crops, 36(8):975-981. (in Chinese)
[23] 朱浩, 牛艳路, 王佩, 等. 小麦矮秆基因的分子检测及遗传效应研究[J]. 分子植物育种, 2018, 16(6):145-151.
ZHU Hao, NIU Yan-lu, WANG Pei, et al.(2018)Molecular Detection and Genetic Effect Research of Dwarf Gene in Wheat[J]. Molecular Plant Breeding, 16(6):145-151. (in Chinese)
[24] Unknown authors. (2014). Genetic effect of dwarfing gene rht13 compared with rht-d1b on plant height and some agronomic traits in common wheat (triticum aestivum l.). Field Crops Research, 162:39-47.

117份小麦种质中5个矮秆基因的分子检测

Molecular Detection of Five Dwarf Genes in 117 Wheat Germplasm Resources

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	陈传信, 张永强, 聂石辉, 孔德鹏, 赛力汗·赛, 徐其江, 雷钧杰. 生物质炭施用量对滴灌冬小麦生长发育和产量的影响[J]. 新疆农业科学, 2023, 60(9): 2146-2151.
[2]	王立红, 张宏芝, 张跃强, 李剑峰, 王重, 高新, 时佳, 王春生, 夏建强, 樊哲儒. 不同产量水平冬小麦产量差异形成的干物质生产、转运及氮肥利用分析[J]. 新疆农业科学, 2023, 60(9): 2152-2162.
[3]	王晓雨, 王小平, 史文宇, 刘美艳, 马健, 郭云鹏, 宋瑞欣, 王清涛. 拔节期冬小麦光合特性、干物质积累和产量对干旱胁迫的响应[J]. 新疆农业科学, 2023, 60(9): 2163-2172.
[4]	杨红梅, 张跃强, 史应武, 吾买尔江·库尔班, 林青, 王宁, 楚敏, 曾军. 不同类型叶面肥喷施对冬小麦籽粒产量和品质的影响[J]. 新疆农业科学, 2023, 60(9): 2182-2188.
[5]	秦鹏亮, 周霄, 王爽, 李佳琪, 刘颖, 张娜, 杨文香. 小麦品种衡观35抗茎基腐病EMS突变及其鉴定[J]. 新疆农业科学, 2023, 60(9): 2231-2238.
[6]	张立国, 郭春贵, 常立春, 郭新磊, 张涛, 武剑, 梁建丽, 高杰, 王晓武. 白菜抗根肿病分子标记开发与种质资源鉴定[J]. 新疆农业科学, 2023, 60(9): 2281-2290.
[7]	董艳雪, 贾永红, 张金汕, 李丹丹, 王凯, 罗四维, 王润琪, 石书兵. 不同生态区环境下春小麦干物质积累及产量形成分析[J]. 新疆农业科学, 2023, 60(8): 1848-1857.
[8]	冯梅, 刘超勤, 陈杰, 刘文龙, 杨志刚, 田杰英, 黄鑫. 小麦不同杂交F₁代性状变异及杂种优势分析[J]. 新疆农业科学, 2023, 60(8): 1858-1865.
[9]	李怀胜, 艾洪玉, 孟玲, 王贺亚, 张磊, 艾海峰. 减氮下运筹养分吸收高峰期追施比例对春小麦的影响[J]. 新疆农业科学, 2023, 60(8): 1866-1872.
[10]	张永强, 陈传信, 聂石辉, 徐其江, 赛力汗·赛, 雷钧杰. 矮壮素滴施时期对冬小麦茎秆抗倒伏能力的调控分析[J]. 新疆农业科学, 2023, 60(8): 1873-1878.
[11]	杨金钰, 王西和, 孙九胜. 水培对全株大麦和小麦苗氨基酸组成的影响及对比分析[J]. 新疆农业科学, 2023, 60(7): 1589-1595.
[12]	田文强, 董艳雪, 史永清, 雷钧杰, 孙刚刚, 王泓懿, 聂凌帆, 郭飞, 艾洪玉, 石书兵, 张金汕. 播期对超晚冬麦茎秆性状及群体动态的影响[J]. 新疆农业科学, 2023, 60(6): 1301-1307.
[13]	董德誉, 程宇坤, 王睿, 雷钧杰, 王伟, 陈传信, 张永强, 耿洪伟. 基于无人机多光谱影像反演不同生育期小麦光合参数分析[J]. 新疆农业科学, 2023, 60(6): 1308-1318.
[14]	张永强, 陈传信, 徐其江, 聂石辉, 雷钧杰, 刘昌文. 氮肥增效剂与氮肥减量配施对冬小麦叶片生理及产量的影响[J]. 新疆农业科学, 2023, 60(6): 1319-1325.
[15]	赖宁, 耿庆龙, 李永福, 李娜, 信会男, 步生兵, 陈署晃. 有机无机配施对超晚播冬小麦产量、氮磷养分吸收利用及土壤肥力的影响[J]. 新疆农业科学, 2023, 60(6): 1335-1343.