新疆农业科学 ›› 2019, Vol. 56 ›› Issue (6): 1104-1111.DOI: 10.6048/j.issn.1001-4330.2019.06.013

• 园艺·林业·生理生化 • 上一篇    下一篇

辣椒炭疽病基因紧密连锁的KASPar标记的开发

赵园园1, 2, 刘议蔚2, 张正海2, 曹亚从2, 于海龙2, 张宝玺2, 高杰1, 王立浩2   

  1. 1.新疆农业大学林学与园艺学院,乌鲁木齐 830052;
    2.中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2019-06-20 发布日期:2019-08-08
  • 通信作者: 王立浩(1972-)男,山东人,研究员,硕士生导师,研究方向为蔬菜学,(E-mail)wanglihao@caas.cn
    高杰(1963-),男,天津人,教授,博士生导师,研究方向为蔬菜学,(E- mail)ofc111@163.com
  • 作者简介:赵园园(1994-),女,河南人,硕士研究生,研究方向为蔬菜学,(E-mail)1027316020@ qq.com
  • 基金资助:
    国家自然科学基金(31572121);中国农业科学院创新工程(CAAS-ASTZP-ZVFCAAS);国家重点研发(2017YFD01019001);农业部大宗蔬菜产业技术体系项目(CARS-25)

Development of KASPar Marker Closely Linked to the Major Gene Resistantto Colletotrichum acutatum in Pepper (Capsicum annuum L.)

ZHAO Yuan-yuan1, 2, LIU Yi-wei2, ZHANG Zheng-hai2, CAO Ya-cong2, YU Hai-long2, MA Wen-wen2, ZHANG Bao-xi2, GAO Jie1, WANG Li-hao2   

  1. 1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Key Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Online:2019-06-20 Published:2019-08-08
  • Correspondence author: Wang Li-hao (1972-), male, native place: Shandong,Researcher, Master tutor, research field:olericulture,(E-mail)wanglihao@caas.cn
    Gao Jie (1963-), male, native place: Tianjing. Professor, Master tutor, research field: olericulture,(E-mail) ofc111@163.com
  • Supported by:
    upported by National Natural Science Foundation of China(31572121); Scientific and Technological Innovation Project of the Chinese Academy of Agricultural Sciences (CAAS-ASTZP-ZVFCAAS); National Key R & D Program of China (2017YFD01019001); Project of China Ministry of Agriculture (CARS-25)

摘要: 【目的】研究辣椒炭疽病抗性基因的遗传规律。在5号连锁群中,定位辣椒炭疽病抗性主效基因AnRGO5,并开发分子标记。【方法】以高抗炭疽病品种PBC932(Capsicum chinense )为父本,以高感炭疽病的中早熟材料77013(Capsicum annuum)为母本杂交产生BC4S1、BC4S2群体,用于辣椒绿熟期抗炭疽病基因定位。选用尖孢炭疽菌为试验用菌,采用显微注射法对绿熟果接病,根据病斑直径进行表型分析。根据抗、感亲本重测序结果,开发与绿熟期抗炭疽病连锁的Kaspar标记。【结果】辣椒果实表面病斑直径呈连续分布,符合数量性状的遗传特点。通过连锁分析,将炭疽病抗性基因AnRGO5定位在5号连锁群的标记P5L-866与标记P5L-259之间,遗传距离2.9 cM。开发的标记P5L-117 与基因紧密连锁,标记准确率93.5%。【结论】在辣椒的BC3S1群体中,将果实绿熟期抗性基因定位于5号染色体的标记区间内。辣椒炭疽病抗性遗传为显性遗传,是由两对主效基因控制的。

关键词: 辣椒; 炭疽病; 尖孢炭疽菌; 基因定位

Abstract: 【Objective】 To study the inheritance of pepper anthracnose resistance gene, Kaspar marker linked with the anthracnose resistance major gene AnRGO5 was identified and developed.【Method】Pepper inbred line PBC932 (Capsicum chinense), resistant to anthracnose (Colletotrichum acutatum) was used as the male parent, and the BC4S1 and BC4S2 populations were obtained by hybridization of the pepper inbred line 77013 (Capsicum annuum) , which was sensitive to anthracnose. The study used Colletotrichum acutatum as the test bacteria, and the green ripe fruit was inoculated by microinjection method, and the phenotypic analysis was carried out according to the diameter of the lesion. Based on the results of re-sequencing of anti- and susceptible parents, Kaspar markers linked to anthracnose in green ripening stage were developed to construct a genetic linkage map.【Result】The diameter of lesions on the surface of pepper fruit was continuous and accorded with the genetic characteristics of quantitative traits. The anthracnose resistance gene AnRGO5 was mapped to the marker P5L-866 of the 5th linkage group and the marker P5L-259 by linkage analysis, and the genetic distance was 2.9 cM. The marker P5L-117 developed was closely linked to the gene and the labeling accuracy was 93.5%.【Conclusion】 In the BC3S1 population of pepper, the fruit green ripening resistance gene was located in the marker interval of chromosome 5. Pepper anthracnose resistance is dominantly hereditary and is controlled by two pairs of major genes.

Key words: pepper; anthracnose; Colletotrichum acutatum; gene mapping

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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