低温胁迫对红加仑枝条渗透物质含量、膜质过氧化及保护酶活性的影响

doi:10.6048/j.issn.1001-4330.2019.04.011

摘要/Abstract

摘要： 【目的】研究引种小浆果红加仑3个品种阿尔泰红宝石、邱阔夫、粉色格兰斯卡亚对低温胁迫的生理响应。【方法】 以1年生离体枝条为试材, 测定不同低温胁迫(-15(对照)、-20、-25、-30、-35和-40℃)处理下3个品种枝条的可溶性糖、可溶性蛋白质、脯氨酸含量、丙二醛和相对电导率以及CAT、SOD、POD的活性。分析低温胁迫对植物枝条生理指标的影响差异,对所得数据进行差异显著性分析和多重比较,并采用主成分分析法对不同品种抗寒性进行综合评价。【结果】(1)随着温度的降低各品种的可溶性糖含量、可溶性蛋白含量、脯氨酸含量、电导率均呈S形变化趋势,MDA含量呈先上、后下再下的变化趋势,阿尔泰红宝石、丘阔夫枝条内 MDA含量随着低温胁迫的加剧,-35和-40℃时呈急剧下降趋势;CAT、SOD、POD的活性呈先升,后降的波浪形变化趋势, 阿尔泰红宝石SOD活性随着低温胁迫的加剧出现逐渐下降趋势,但均高于其余两个品种。(2)红加仑3个品种抗寒性综合得分排序是阿尔泰红宝石＞粉红戈兰斯卡亚＞邱阔夫。【结论】在低温胁迫条件下,阿尔泰红宝石、粉红戈兰斯卡亚、邱阔夫等红加仑品种,能通过提高渗透调节物质含量和增强保护酶活性来缓解胁迫造成的过氧化伤害,表现出较强的抗寒能力,相对电导率和主成分分析的综合评价均能准确鉴定红加仑的抗寒性。

关键词: 红加仑; 低温胁迫; 品种; 主成分分析

Abstract: 【Objective】 To study the physiological response of three introduced small berry red gallons varieties: Altayskaya rubinaya, Zhukov and Golandiskaya rozavoya to low temperature stress.【Method】Taking the one year branches as the experimental materials, and soluble sugar, soluble protein, proline content, MDA, relative electrolyte conductivity CAT, SOD and POD contents were investigated on varieties of Ribes rubrum branches under the low temperature stress (-15℃(CK), -20℃, -25℃, -30℃, -35℃ and -40℃)and the effects of low temperature stress on the physiological indexes of plant branches were discussed, and the significant differences and multiple comparisons of the obtained data were carried out, and the cold resistance of different varieties was comprehensively evaluated by principal component analysis (PCA).【Result】(1) The soluble sugar, soluble protein, proline, and relative electrolyte conductivity were increased by low temperature stress following an S-curve. MDA content increased at the beginning and then decreased, Since the low temperature stress, the MDA of Altayskaya rubinaya, Zhukov sharply decreased at the -30℃ and -40℃; The CAT, SOD, POD content increased at the beginning and then decreased, Since the low temperature stress, the SOD of Altayskaya rubinaya Gradually decreased, but higher than another two varieties. (2) Comprehensive scores and the sorting of different verities of Ribes rubrum were: Altayskaya rubinaya ＞ Golandiskaya rozavoya ＞Zhukov. 【Conclusion】 Under the condition of low temperature stress, the different varieties of Ribes rubrum could improve the osmotic regulation substances content and enhance the protective enzyme activity of peroxide damage to relieve stress, which showed stronger cold resistance ability, the relative conductivity and comprehensive evaluation of principal component analysis can accurately identify the cold resistance of Ribes rubrum.

Key words: Ribes rubrum; low temperature stress; varieties; principal component analysis

中图分类号:

S623.9

古丽江·许库尔汗, 孙雅丽, 阿依古丽·铁木儿, 卢明艳, 哈尔肯·叶尔江 , 巴哈尔古丽·阿尤甫. 低温胁迫对红加仑枝条渗透物质含量、膜质过氧化及保护酶活性的影响[J]. 新疆农业科学, 2019, 56(4): 685-695.

Gulijiang Xukuerhan, SUN Ya-li ,Ayiguli Tiemuer, LU Ming-yan, Haerken Yeerjiang, Baharguli Ayoufu. Effects of Low Temperature Stress on the Content of Penetration Substance, Membrane Peroxidation and Protective Enzyme Activity in Ribes rubrum [J]. Xinjiang Agricultural Sciences, 2019, 56(4): 685-695.

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