新疆农业科学 ›› 2019, Vol. 56 ›› Issue (2): 317-324.DOI: 10.6048/j.issn.1001-4330.2019.02.013

• • 上一篇    下一篇

新疆棉田根际土壤真菌荧光PCR技术定量及其时空动态分析

党文芳1,2, 李雪艳1,2, 杨红梅2,3, 楚敏2,3, 高雁2,3, 曾军2,3, 霍向东2,3, 张涛2,3, 林青2,3, 欧提库尔2,3, 李玉国2,3, 娄恺2,3, 史应武1,2,3,4   

  1. 1.新疆大学生命科学与技术学院,乌鲁木齐 830052;
    2.新疆农业科学院微生物应用研究所 ,乌鲁木齐830091;
    3.新疆特殊环境微生物实验室,乌鲁木齐 830091;
    4.农业农村部西北绿洲农业环境重点实验室,乌鲁木齐830091
  • 收稿日期:2019-01-03 出版日期:2019-02-20 发布日期:2019-05-22
  • 通信作者: 史应武(1973-),男,甘肃天水人,博士研究员,研究方向为农业有害生物控制,(E-mail)syw1973@126.com
  • 作者简介:党文芳(1994-),女,甘肃武威人,硕士研究生,研究方向为食品生物技术,(E-mail)1954358900@qq.com
  • 基金资助:
    国家自然科学基金(41471220);新疆维吾尔自治区优秀青年科技人才培养(qn2005yx023);中国博士后科学基金(2016M602953XB)

Quantitative and Spatio-Temporal Dynamic Analysisof Rhizosphere Soil Fungi by PCR Technology in Xinjiang Cotton Fields

DANG Wen-fang1,2, LI Xue-yan1,2, YANG Hong-mei2,3, CHU Ming2,3, GAO Yan2,3, ZENG Jun2,3, HUO Xiang-dong2,3, ZHANG Tao2,3, LIN Qing2,3, Outikuer2,3, LI Yu-guo2,3, LOU Kai2,3, SHI Ying-wu1,2,3,4   

  1. 1.College of Life Sciences and Technology, Xinjiang University, Urumqi 830052, China;
    2.Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
    3.Xinjiang Special Environmental Microbiology Laboratory, Urumqi 830091, China;
    4.Key Laboratory of Northwest Oasis Agricultural Environment, Ministry of Agriculture, Urumqi 830091, China
  • Received:2019-01-03 Online:2019-02-20 Published:2019-05-22
  • Correspondence author: SHI Ying-wu(1973-), Gansu Tianshui, researcher, research direction: Agricultural pest control, (E-mail):syw1973@126.com
  • Supported by:
    the National Natural Science foundation of China (41471220); Excellent Youth Science and Technology Talents Training Project of Xinjiang Uygur Autonomous Region (qn2005yx023); China Postdoctoral Science Foundation Project (2016M602953XB)

摘要: 【目的】研究新疆棉花黄萎病病株根际土壤真菌数量的时空动态变化,及棉花根际土壤真菌与黄萎病病原菌数量的相关性。【方法】用荧光定量PCR方法,测定新疆不同植棉区及其不同生育时期棉田根际土壤真菌总量和棉花黄萎病病原菌数量进行测定,分析棉花黄萎病病株根际土壤真菌数量在不同植棉区和不同生育时期的变化趋势,及棉花根际土壤真菌与棉花黄萎病病原菌数量的相关性。【结果】新疆不同植棉区的棉花在不同生育期其病株根际土壤真菌数量表现出不同变化趋势。哈密棉花病株吐絮期根际土壤真菌最大值达 6.16×104 copies/g FRW;库尔勒棉花病株根际土壤真菌在苗期达到最大为4.23×104 copies/g FRW;阿拉尔棉花病株根际土壤真菌在苗期至蕾期逐渐增加,随后逐渐减小,在吐絮期达到最小值为1.41×10-4copies/g FRW。北疆精河和东疆哈密棉花根际土壤真菌数量与黄萎病病原菌数量的PCC分别高达0.989和0.993,呈显著正相关。南疆图木舒克棉花根际土壤真菌数量与黄萎病病原菌数量的PCC为0.880,呈正相关。【结论】新疆棉花黄萎病病株根际土壤真菌含量较高,在不同采样区和不同生育期,其根际土壤真菌总量均呈波动性变化。从棉花的四个生育期(苗期、蕾期、花铃期、吐絮期)来看,棉花病株根际土壤真菌数量最大值出现在吐絮期。新疆棉花病株根际土壤真菌数量的空间变化是东疆大于南疆大于北疆。在不同生态区,棉花根际土壤真菌和棉花黄萎病病原菌之间表现为正相关,而在不同发育期则表现为负相关。

关键词: 棉花; 根际土壤真菌; TaqMan探针; 荧光定量PCR; 时空动态变化

Abstract: 【Objective】 To study the spatio-temporal dynamics of the number of fungi in the rhizosphere soil of cotton Verticillium wilt strains in Xinjiang, and the correlation between them. 【Methods】 Fluorescence quantitative PCR was used to determine the total amount of fungi in the rhizosphere soil and the number of pathogens in different cotton-growing areas and in different growth stages in Xinjiang and to analyze their variation trend and the correlation between cotton rhizosphere soil fungi and the number of cotton Verticillium pathogens. 【Results】 The number of fungi in the rhizosphere soil of different cotton growing areas and different growth stages in Xinjiang showed different trends. The maximum value of fungi in rhizosphere soil of Hami cotton was 6.16×104 copies/g FRW; the rhizosphere soil fungi of Korla cotton diseased strains reached the maximum at seedling stage, which was 4.23×104 copies/g FRW; the rhizosphere soil fungi of Aral cotton diseased strains increased gradually from the seedling stage to the bud stage, then decreased gradually, and reached a minimum at the flocculation stage, which was 1.41×10-4copies/g FRW. There was a significant positive correlation between the number of soil fungi and the number of the pathogens of the Verticillium dahliae in Jinghe in northern Xinjiang and in Hami in Eastern Xinjiang, and their PCC were as high as 0.989 and 0.993, respectively. The number of fungi in the rhizosphere soil of Tumushuke cotton in southern Xinjiang was positively correlated with the number of pathogens of Verticillium dahliae, which was 0.880.【Conclusion】 The content of fungi in rhizosphere soil of cotton Verticillium wilt was higher, and in different sampling areas and different growth stages, the total amount of soil fungi in rhizosphere fluctuated. In terms of the four growth stages of cotton (seedling stage, bud stage, flowering stage, boll opening stage), the maximum number of the soil fungi in the rhizosphere of the plant disease appeared in the boll opening period. In view of the comprehensive cotton planting areas, the spatial variation of the number of fungi in the rhizosphere soil of cotton diseased plants in Xinjiang was Eastern Xinjiang > Southern Xinjiang > Northern Xinjiang. There was a positive correlation between cotton rhizosphere soil fungi and cotton Verticillium pathogens in different ecological regions of cotton, but, on the other hand, negative correlation was observed at different developmental stages.

Key words: cotton; Rhizosphere soil fungus; TaqMan probe; fluorescence quantitative PCR; spatio-temporal dynamics

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
地址:乌鲁木齐市南昌路403号新疆农业科学院
邮编:830091
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E-mail:xjnykx-h@xaas.ac.cn


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