一氧化氮对低温贮藏桃果实线粒体氧化损伤的影响

doi:10.6048/j.issn.1001-4330.2018.12.001

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (12): 2157-2165.DOI: 10.6048/j.issn.1001-4330.2018.12.001

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一氧化氮对低温贮藏桃果实线粒体氧化损伤的影响

胡珊¹,叶志恒²,冯建荣¹,朱树华²

1.石河子大学农学院园艺系/特色果树栽培生理与种质资源利用兵团重点实验室,新疆石河子 832003;
2.山东农业大学化学与材料科学学院, 山东泰安 271018

收稿日期:2018-09-26 发布日期:2019-04-18
通信作者: 冯建荣(1969-),女,新疆石河子人,教授,博士生导师,研究方向为果树遗传育种与种质资源创新利用,(E-mail)fengjr102@126.com
朱树华(1978-),男,山东泰安人,教授,硕士生导师,研究方向为化学生物学,(E-mail)shuhu@sdau.edu.cn
作者简介:胡珊(1993-),女,新疆昌吉人,硕士研究生,研究方向为果树种质资源评价与利用,(E-mail)15299962580@163.com
基金资助:
国家自然科学基金项目(31270723;31470686)

Effects of Nitric Oxide on Mitochondrial Oxidative Damage in Peaches Stored at Low Temperature

HU Shan¹, YE Zhi-heng², FENG Jian-rong¹,ZHU Shu-hua²,

1.College of Agronomy, Shihezi University /Key Laboratory of Key Laboratory of Cultivation Physiology and Germplasm Resources Utilization of Featured Fruits and Vegetables of Xinjiang Production and Construction Corps,Shihezi Xinjiang 832003,China;
2. College of Chemistry and Material Science, Shandong Agricultural University, Tai'an, Shandong 271018, China

Received:2018-09-26 Published:2019-04-18
Correspondence author: Feng Jian-rong (1969-), Shihezi, Xinjiang, Professor, Doctoral tutor, Research direction: Genetic Breeding of Fruit Trees and Innovative Utilization of Germplasm Resources, (E-mail) fengjr102@126.com
ZHU Shu-hua (1978-), Taian, Shandong, Professor, Master tutor, Research direction: Research of chemicobiology, (E-mail) shuhua@sdau.edu.cn
Supported by:
The National Natural Science Foundation of China (31270723； 31470686）

摘要/Abstract

摘要： 【目的】分析一氧化氮(NO)对冷藏过程中桃果实线粒体氧化损伤的影响。【方法】分别用15 μmol/L NO、5 μmol/L c-PTIO (NO清除剂)、对照(去离子水)浸泡30 min处理桃果实。测定0℃下桃果实的线粒体完整性、活性氧含量、抗氧化酶的活性及相关品质指标。【结果】在桃果实低温冷藏过程中,NO处理可以维持桃果实硬度、色差L值及可溶性固形物含量,降低线粒体耗氧量和减少活性氧的积累,调节线粒体呼吸控制率(RCR)、线粒体膜电势,线粒体细胞色素C含量以及线粒体抗氧化酶活性。【结论】NO通过减少线粒体氧化损伤,维持线粒体的完整性,延长桃果实的贮藏期。

关键词: 桃; 一氧化氮; 线粒体; 活性氧; 抗氧化酶

Abstract: 【Objective】 To study the effects of nitric oxide (NO) on mitochondrial oxidative damage in peach fruit stored at 0℃.【Method】Peaches were treated with 15 μmol/L NO, 5 μmol/L c-PTIO (NO scavenger) and control to measure the related quality indexes of peach fruits, at 0 ℃ such as the mitochondrial integrity, the content of reactive oxygen species (ROS) and the antioxidant enzyme activity peaches.【Result】NO could maintain the firmness, decline the L value and the content of soluble solid of peaches, reduce the oxygen consumption and the content of ROS, regulate the mitochondrial respiratory control rate (RCR), mitochondrial membrane potential, the content of mitochondrial cytochrome C and the activity of antioxidant enzyme during the storage eperiod.【Conclusion】NO can maintain the mitochondrial integrity by decreasing mitochondrial oxidative damage, so as to prolong the storage of peaches.

Key words: peach (Prunus persica); nitric oxide (NO); mitochondria; reactive oxygen species (ROS); antioxidant enzymes

中图分类号:

S609

胡珊, 叶志恒, 冯建荣, 朱树华. 一氧化氮对低温贮藏桃果实线粒体氧化损伤的影响[J]. 新疆农业科学, 2018, 55(12): 2157-2165.

HU Shan, YE Zhi-heng, FENG Jian-rong, ZHU Shu-hua, . Effects of Nitric Oxide on Mitochondrial Oxidative Damage in Peaches Stored at Low Temperature[J]. Xinjiang Agricultural Sciences, 2018, 55(12): 2157-2165.

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一氧化氮对低温贮藏桃果实线粒体氧化损伤的影响

Effects of Nitric Oxide on Mitochondrial Oxidative Damage in Peaches Stored at Low Temperature

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