不同培养基对春小麦×玉米单倍体胚分化成苗的影响

doi:10.6048/j.issn.1001-4330.2018.11.004

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (11): 1988-1993.DOI: 10.6048/j.issn.1001-4330.2018.11.004

• 作物遗传育种·种质资源·分子生物学 • 上一篇下一篇

不同培养基对春小麦×玉米单倍体胚分化成苗的影响

王重^1,2, 樊哲儒^1,2, 张跃强^1,2, 李剑峰^1,2, 高新^1,2, 王子霞¹

1.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091;
2.农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐 830091

收稿日期:2018-09-15 发布日期:2019-02-22
通信作者: 樊哲儒(1964-),男,甘肃民勤人,研究员,研究方向为小麦遗传育种,E-mail:fzr518@163.com
作者简介:王重(1982-),男,陕西长安人,副研究员,研究方向为小麦遗传育种,E-mail:wangzhongac@126.com
基金资助:
新疆维吾尔自治区青年科学基金项目(2013211B23);新疆农业科学院青年科学基金项目(xjnkq-2013025)

Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

WANG Zhong^1,2, FAN Zhe-ru^1,2, ZHANG Yue-qiang^1,2, LI Jian-feng^1,2, GAO Xin^1,2, WANG Zi-xia¹

1.Research Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region, Ministry of Agriculture, P.R. China, Urumqi 830091, China

Received:2018-09-15 Published:2019-02-22
Correspondence author: FAN Zhe-ru(1964-),male, Minqin of Gansu Province, Researcher, Research focuses on wheat genetics and breeding, E-mail:fzr518@163.com
Supported by:
Youth Science Fund Projects of Xinjiang Uygur Autonomous Region (2013211B23); Youth Science Fund Projects of Xinjiang Academy of Agricultural Sciences(xjnkq-2013025)

摘要/Abstract

摘要： 【目的】筛选适宜春小麦单倍体胚分化成苗的培养基,获得稳定且高效的单倍体胚培养方法。【方法】分别使用8种不同配方培养基对春小麦单倍体胚进行离体培养,使其分化成苗,根据成苗率及生长情况筛选培养基。【结果】在不添加激素的1/2 MS培养基中春小麦单倍体胚成苗率最高(73.49%),在1/2MS+0.1 mg/L 2,4-D培养基中春小麦单倍体胚能够一步成苗,且生长健壮,根系发达。【结论】1/2 MS培养基较B5培养基更适宜春小麦单倍体胚的分化成苗,2,4-D对于春小麦单倍体胚的根系发育具有明显的促进作用,2,4-D>IAA,1/2 MS+0.1 mg/L 2,4-D培养基是春小麦单倍体胚分化成苗的适宜培养基。

关键词: 春小麦; 单倍体胚; 分化; 培养基

Abstract: 【Objective】 This project aims to screen suitable medium for differentiation of spring wheat haploid embryos and obtain a stable and efficient method.【Method】The haploid embryos were differentiated into plantlets using 8 different mediums,and the mediums was screened according to the seedling rate and growth conditions. 【Result】The seedling rate of spring wheat haploid embryos was the highest (73.49%) in 1/2 MS medium without hormones. The haploid embryos were induced one-step-seedling in 1/2 MS + 0.1 mg/L 2,4- D medium, with stronger plantlets and their roots developed well. 【Conclusion】The 1/2 MS medium was more suitable for the differentiation of spring wheat haploid embryos than the B5 medium, and the 2,4-D had more significant effect on the development of roots and differentiation of embryos. The medium of 1/2 MS + 0.1 mg/L 2, 4-D was a suitable medium for haploid embryos differentiation of spring wheat.

Key words: wheat; haploid embryo; differentiation; medium

中图分类号:

S512

王重, 樊哲儒, 张跃强, 李剑峰, 高新, 王子霞. 不同培养基对春小麦×玉米单倍体胚分化成苗的影响[J]. 新疆农业科学, 2018, 55(11): 1988-1993.

WANG Zhong, FAN Zhe-ru, ZHANG Yue-qiang, LI Jian-feng, GAO Xin, WANG Zi-xia. Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross[J]. Xinjiang Agricultural Sciences, 2018, 55(11): 1988-1993.

参考文献

[1] Zenkteler M, Nitzsche W. (1984). Wide hybridization experiments in cereals. Tag.theoretical & Applied Genetics.theoretische Und Angewandte Genetik, 68(4): 311-315.
[2] Laurie D A, Bennett M D. (1986). Wheat×maize hybridization[J]. Canadian Journal of Genetics and Cytology, 28(2): 313-316.
[3] Laurie D A, Bennett M D. (1988). The production of haploid wheat plants from wheat x maize crosses[J]. Theoretical & Applied Genetics, 76(3): 393-397.
[4] Laurie D A, Reymondie S. (1991). High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize[J].Plant Breeding, 106(3): 182-189.
[5] 陈新民,徐惠君,周俊芳,等. 提高小麦×玉米胚培养植株产生频率的研究[J].中国农业科学, 1996, 29(4): 29-32.
CHEN Xin-ming, XU Hui-jun, ZHOU Jun-fang, et al. (1996). A Study on the increasing frequences of plant production during embryo culture in crosses between wheat and maize [J]. Scientia Agricultura Sinica,29(4): 29-32. (in Chinese)
[6] 闫贵云,左静静,冯瑞云,等. 玉米花粉诱导小麦单倍体技术研究进展[J]. 山西农业科学, 2015,43(9): 1 204-1 206.
YAN Gui-yun, ZUO Jing-jing, FENG Rui-yun, et al. (2015). The research progress of wheat haploid induced by maize pollen [J]. Journal of Shanxi Agricultural Sciences, 43(9): 1,204-1,206. (in Chinese)
[7] 王重,樊哲儒,张跃强,等. 春小麦单倍体胚得胚率的影响因素分析[J]. 新疆农业科学, 2016, 53(8): 1 404-1 408.
WANG Zhong, FAN Zhe-ru, ZHANG Yue-qiang, et al. (2016). Analysis of influencing factors on embryo rate of haploid embryos in spring wheat[J]. Xinjiang Agricultural Sciences, 53(8): 1,404-1,408. (in Chinese)
[8] 孙敬三,刘辉,路铁刚,等. 利用小麦×玉米获得小麦单倍体[J]. 植物学报, 1992,34(11): 817-821.
SUN Jing-san, LIU Hui, LU Tie-gang, et al. (1992) The production of haploid wheat plants via wheat×maize hybridization [J]. Acta Botanica Sinica,34(11): 817-821. (in Chinese)
[9] 陈新民,王凤菊,李思敏,等. 小麦与玉米杂交产生小麦单倍体与双单倍体的稳定性[J]. 作物学报, 2013,39(12): 2 247-2 252.
CHEN Xin-ming, WANG Feng-ju, LI Si-min, et al. (2013). Stable production of wheat haploid and doubled haploid by wheat × maize cross [J]. Acta Agronomica Sinica, 39(12): 2,247-2,252. (in Chinese)
[10] Niroula R K, Bimb H P. (2009). Overview of wheat × maize system of crosses for dihaploid induction in wheat[J]. World Applied Sciences Journal, 7(8): 1,037-1,045.
[11] Brazauskas G, Pa akinskien I, Ruzgas V. (2005). Improved approaches in wheat × maize crossing for wheat doubled haploid production[J]. Biologija, (4): 15-18.
[12] 赵翠荣,杨青,王立峰,等. 培养基和基因型对小麦×玉米诱导单倍体效率的影响[J]. 中国种业, 2014,(9): 33-35.
ZHAO Cui-rong, YANG Qing, WANG Li-feng, et al. (2014). Effects of mediums and genotypes on frequencies of producing haploid of wheat×maize [J]. China Seed Industry, (9): 33-35. (in Chinese)
[13] 张阔,胡洪林,夏艳龙,等. 培养基对小麦×玉米诱导小麦单倍体效率的影响[J]. 现代化农业, 2009,(9): 9-11.
ZHANG Kuo, HU Hong-lin, XIA Yan-long, et al. (2009). Effects of mediums and genotypes on frequencies of producing haploid of wheat×maize [J]. Modernizing Agriculture, (9): 9-11. (in Chinese)
[14] 蔡东明,吉万全,任志龙,等. 小麦单倍体育种中培养基的研究[J]. 中国农学通报, 2007,23(5): 71-74.
CAI Dong-ming, JI Wan-quan, REN Zhi-long, et al. (2007). Studies on culture medium of wheat haploid breeding[J]. Chinese Agricultural Science Bulletin, 23(5): 71-74. (in Chinese)
[15] 陈淑萍,王雪征,崔银辉,等. 不同培养基对小麦×玉米杂种胚离体培养成苗的影响[J]. 核农学报, 2013,27(6): 758-762.
CHEN Shu-ping, WANG Xue-zheng, CUI Yin-hui, et al. (2013). Effect of different culture media on haploid plantlets production from the wheat×maize cross[J]. Acta Agriculturae Nucleatae Sinica, 27(6): 758-762. (in Chinese)
[16] 高艳,唐建卫,殷贵鸿,等. 培养基对小麦单倍体胚成苗率的影响[J]. 中国农学通报, 2015,31(21):107-110.
GAO Yan, TANG Jian-wei, YIN Gui-hong, et al. (2015). Effect of culture media on seedling rate of wheat haploid[J]. Chinese Agricultural Science Bulletin, 31(21):107-110. (in Chinese)

不同培养基对春小麦×玉米单倍体胚分化成苗的影响

Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

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