一种侵染红枣叶片和果实的病原真菌鉴定

doi:10.6048/j.issn.1001-4330.2018.09.014

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (9): 1682-1688.DOI: 10.6048/j.issn.1001-4330.2018.09.014

• 植物保护·土壤肥料·农业生态环境 • 上一篇下一篇

一种侵染红枣叶片和果实的病原真菌鉴定

萨吉达木·艾则孜, 麦合木提江·米吉提, 赵志强, 许泰百, 郭庆元

新疆农业大学,乌鲁木齐 830052

收稿日期:2018-07-18 出版日期:2018-09-20 发布日期:2018-12-07
通信作者: 郭庆元(1962-),四川人,教授,博士,研究方向为植物病理,(E-mail)guoqingyuan3009@sina.com
作者简介:萨吉达木·艾则孜(1992-),新疆人,硕士研究生,研究方向为植物病理,(E-mail)1539475398@qq.com
基金资助:
新疆维吾尔自治区科技计划项目“新疆特色果树高效安全生产关键技术研究集成与示范”(201130102);新疆农业大学研究生科研创新项目(XJAUGRI16-019)

Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

Sajidamu Aizezi¹, Mahemutijiang MIjiti¹, ZHAO Zhi-qiang¹, XU Tai-bai¹, GUO Qing-yuan¹

College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2018-07-18 Online:2018-09-20 Published:2018-12-07
Correspondence author: GUO Qing-yuan(1962-),Sichuan people, Professor of college of Agronomy, Xinjiang Agricultural University, working on plant pathology,(E-mail)guoqingyuan3009@sina.com
Supported by:
Science and Technology Planning project of Xinjiang Uygur Autonomous Region " Integration and demonstration of Key Technologies Efficient and Safe Production of Featured Fruit Trees in Xinjiang" (201130102);Scientific Research Innovation Project for Postgraduate Students of Xinjiang Agricultural University(XJAUGRI16-019)

摘要/Abstract

摘要： 【目的】研究新疆地区近年发生的一种与枣花叶病有密切关系的病原真菌。【方法】通过病样采集、病原菌分离、回接证病,以及形态观察和rDNA-ITS序列比对等方法,鉴定病原菌。【结果】从有明显花叶症状和褐色叶斑的病叶以及有畸果症状和褐色斑点的病果中分离获得多个真菌单孢菌株,用针刺法将其接种到枣叶和幼果后出现与田间症状基本相似的褐色坏死斑,确定其对枣叶、果的致病性;该菌病原菌形态特征与头状茎点霉基本一致,3个分离菌株的rDNA-ITS序列与头状茎点霉的同源性达到99%。【结论】根据病原菌的形态特征和分子鉴定结果,将该病原菌鉴定为头状茎点霉(Peyronellaea. glomerata)。

关键词: 红枣; 褐色坏死斑; 头状茎点霉; 形态特征; 分子鉴定

Abstract: 【Objective】 In order to clarify the recent occurrence of a species of pathogenic fungi that is closely related to Jujube mosaic disease in Xinjiang.【Method】The pathogen was identified by collecting disease samples, isolating pathogenic bacteria, recovering syndromes, morphological observation and rDNA-ITS sequence alignment.【Result】The spore strains were isolated by some significant mosaic and brown leaf spot samples and abnormal fruit samples. After inoculation of jujube leaves and young fruits with acupuncture, brown necrotic spots similar to the field symptoms were found, and the pathogenicity of them to the leaves and fruits of jujube was determined. The morphological characteristics of the pathogen were basically the same as that of Peyronellaea glomerata, and the homology between the rDNA-ITS sequence of three isolates and that of Peyronellaea glomerata was 99%.【Conclusion】On the basis of morphological and ITS sequence information, the pathogen of the new disease of jujube was identified as Peyronellaea glomerata in Xinjiang.

Key words: Jujube; brown necrosis spot Peyronellaea glomerata; morphology; molecular identification

中图分类号:

S665.1
S188

萨吉达木·艾则孜, 麦合木提江·米吉提, 赵志强, 许泰百, 郭庆元. 一种侵染红枣叶片和果实的病原真菌鉴定[J]. 新疆农业科学, 2018, 55(9): 1682-1688.

Sajidamu Aizezi, Mahemutijiang MIjiti, ZHAO Zhi-qiang, XU Tai-bai, GUO Qing-yuan. Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1682-1688.

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一种侵染红枣叶片和果实的病原真菌鉴定

Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

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