红枣黑斑病拮抗细菌JK1产芽孢培养基的优化

doi:10.6048/j.issn.1001-4330.2018.06.013

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (6): 1098-1106.DOI: 10.6048/j.issn.1001-4330.2018.06.013

红枣黑斑病拮抗细菌JK1产芽孢培养基的优化

王宁¹, 侯敏¹, 房世杰², 艾尼江·尔斯满¹, 侯新强¹, 杨蓉¹, 包慧芳¹

1.新疆农业科学院微生物应用研究所,乌鲁木齐 830091;
2.新疆农业科学院科研管理处,乌鲁木齐 830016;

收稿日期:2018-01-16 发布日期:2018-08-31
通信作者: 包慧芳(1978-),女,湖南岳阳人,副研究员,研究方向为微生物工程,(E-mail)779556527@qq.com
作者简介:王宁(1979-)女,山东人,副研究员,研究方向为微生物发酵,(E-mail)150700126@qq.com
基金资助:
自治区自然科学基金项目“南疆红枣黑斑病病原菌分离及高效拮抗菌筛选”(2015211A031)

The Optimization of Fermentation Medium for Antagonistic Bacteria JK1 against Jujube Black Rot

WANG Ning¹, HOU Min¹, FANG Shi-jie², Ghenijan Osman¹, HOU Xin-qiang¹, YANG Rong¹, BAO Hui-fang¹

1.Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. Office of Scientific Research Affairs, Xinjiang Academy of Agricultural Sciences, Urumqi 830016, China;

Received:2018-01-16 Published:2018-08-31
Correspondence author: BAO Hui-fang (1978- ),female, native place: Yueyang, Hunan. Associate research fellow, research field: Microbial engineering. (E-mail)779556527@qq.com
Supported by:
The Natural Science Foundation of Xinjiang Uygur Autonomous Region "Isolation ofPathogenic Bacteria of Jujube Black Rot and Screening of High Efficiency Antagonistic Strains in Southern Xinjiang" (2015211A031)

摘要/Abstract

摘要： 【目的】 采用响应面法对红枣黑斑病的拮抗细菌JK1培养基进行优化,提高其发酵产物中芽孢的浓度。为该菌应用提供数据。【方法】单因素实验确定培养基碳氮源物质,Plackett-Burman试验分析发酵培养基中对JK1发酵影响最重要的主要因素;运用最陡爬坡法对主要因素进行试验,获得主要因素的最适范围。通过响应面分析得到主要因素的最优水平。【结果】优化后的培养基组成为:麦芽浸粉 25.00 g/L、葡萄糖 0.57 g/L、大豆蛋白胨 12.5 g/L、NaCl 2.7 g/L、K₂HPO₄ 0.25 g/L 、MgSO₄ 0.625 g/L。【结论】在最优发酵培养基培养下,多粘类芽孢杆菌的芽孢数提高了3.4倍,达到4.92×10⁹CFU/mL。

关键词: 红枣黑斑病; 多粘类芽孢杆菌; 芽孢产量; 培养基优化,响应面试验

Abstract: 【Objective】 The project aims to optimize the fermentation media of antagonistic bacteria JK1 against jujube black rot by response surface methodology in the hope of improving the concentration of spores in flask culture conditions.【Method】After determining the carbon and nitrogen source of the medium by single factor experiment, the most important factors affecting the fermentation of JK1 in the fermentation medium were analyzed by the Plackett Burman test and then the steepest slope method was used to test the main factors and the optimum range of the main factors was obtained. Finally, the optimal level of the main factors was ascertained by response surface analysis.【Result】The optimal formulation of fermentation medium was: malt extract 25.00 g/L, glucose 0.57 g/L, soya peptone 12.5 g/L, NaCl 2.7 g/L, K₂HPO₄ 0.25 g/L and MgSO₄ 0.62g/L.【Conclusion】JK1 was cultured by the optimized fermentation medium, and the count of spore increased 3.4 times and reached 4.92 × 10⁹ CFU/mL., which had provided data for the further application of Bacillus polymyxides.

Key words: jujube black rot; Paenibacillus polymyxa; spore production; fermentation medium optimization; response surface test

中图分类号:

王宁, 侯敏, 房世杰, 艾尼江·尔斯满, 侯新强, 杨蓉, 包慧芳. 红枣黑斑病拮抗细菌JK1产芽孢培养基的优化[J]. 新疆农业科学, 2018, 55(6): 1098-1106.

WANG Ning¹, HOU Min¹, FANG Shi-jie², Ghenijan Osman¹, HOU Xin-qiang¹, YANG Rong¹, BAO Hui-fang¹. The Optimization of Fermentation Medium for Antagonistic Bacteria JK1 against Jujube Black Rot[J]. Xinjiang Agricultural Sciences, 2018, 55(6): 1098-1106.

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红枣黑斑病拮抗细菌JK1产芽孢培养基的优化

The Optimization of Fermentation Medium for Antagonistic Bacteria JK1 against Jujube Black Rot

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